Experimental study on bone defect repair by BMSCs combined with a light-sensitive material: g-C3N4/rGO

摘要

Bone marrow mesenchymal stem cells (BMSCs), as seed cells, have played an important role in bone defect repair. However, efficiently amplifying and inducing BMSCsin vitroor vivo remains an urgent problem to be solved. Electrical stimulation has been beneficial to the proliferation and differentiation of BMSCs, but current electrical stimulation methods have a critical disadvantage in that they usually burn the skin. g-C3N4/rGO, a new photosensitive material, can produce photocurrent under natural light irradiation, thus reducing energy consumption. Our purpose was to explore whether this photocurrent can promote the proliferation and differentiation of BMSCs. g-C3N4/rGO synthesised under high temperature and pressure had negligible cytotoxicity as confirmed by methyl thiazolyl tetrazolium to BMSCs. Better osteogenesis was found in the blue light material group than in the light-shielding material group, exhibited by alizarin red staining, alkaline phosphatase activity, Western-Blot, and RT-qPCR. Animal experiments showed that the bone repair potential of the material group was significantly higher than that of the non-material group. Overall, we conclude that g-C3N4/rGO is a new non-toxic photosensitive material which can rapidly induce BMSCs into osteoblasts, accelerating bone regeneration and providing us with a feasible method of rapid bone repair.

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