Effects of Bacillus subtilis and coccidiosis vaccine on growth indices and intestinal microbiota of broilers

作者：Cai, Haiming; Luo, Shengjun; Zhou, Qingfeng; Yan, Zhuanqiang; Liu, Qihong; Kang, Zhen; Liao, Shenquan; Li, Juan; Lv, Minna; Lin, Xuhui; Hu, Junjing; Yu, Shuilan; Zhang, Jianfei; Qi, Nanshan; Sun, Mingfei

来源：Poultry Science, 2022, 101(11): 102091.

DOI：10.1016/j.psj.2022.102091

• Bacillus subtilis
• coccidiosis vaccine
• growth performance
• histopathology
• Intestinal microbiota

Avian coccidiosis is the most serious par-asitic disease in the poultry industry. Therefore, the aim of the current study was to explore the effects of B. sub-tilis and live coccidiosis vaccine alone or in combination on the production performance and anticoccidiosis, as well as the dynamic changes of intestinal microbial com-munity. Nine hundred ninty Mahuang chickens were randomized into 4 preimmune groups including control group, coccidiosis vaccine immunization group; B. subti-lis administration group and a group that was adminis-trated a combination of live coccidiosis vaccine and B. subtilis group. Intestinal mucosal scraps collected from all these experimental groups at the age of 8 d and 15 d for microbial community 16S rRNA gene sequencing. At the age of 25 d, 30 broilers from each preimmune group were randomly assigned to a subgroup infected with Eimeria spp. and renamed as CI, V-CI BS-CI, and VBS-CI group. The production performance was monitored at the age of 25 d, 35 d, 45 d, and 55 d for the rest broilers from each pre-immune group. Otherwise, in the Eimeria spp. challenge stage, intestinal mucosal scraps collected for microbial community sequencing, while duodenum, jejunum, and cecum collected for pathologi-cal examination after sacrifice at the age of 32 d. In addi-tion, the oocysts per gram of feces (OPG) and intestinal lesion score of broilers after Eimeria spp. challenge were also counted. Overall, the probiotics and coccidiosis vac-cine resulted in the significantly improvement of the pro-duction performance. Otherwise, the intestinal lesion score and OPG after Eimeria spp. infection was signifi-cantly decreased in the VBS-CI group (P < 0.05). More-over, these protective effects may also be closely related to genus such as Romboutsia, Blautia, and Butyricococ-cus, as well as microbiota functions like the quorum sensing pathway. According to these results, a combina-tion of B. subtilis and coccidiosis vaccines can improve performance and provide additional protection against Eimeria spp. infection.

• 单位
  广东省农业科学院