The Antiviral Effects of Jasminin via Endogenous TNF-α and the Underlying TNF-α-Inducing Action

作者：Zhu, Xiaohong; Hu, Ziwei; Yu, Tian; Hu, Hao; Zhao, Yunshi; Li, Chenyang; Zhu, Qinchang; Wang, Mingzhong; Zhai, Peng; He, Longxia; Rajoka, Muhammad Shahid Riaz; Song, Xun^*; He, Zhendan^*

来源：MOLECULES, 2022, 27(5): 1598.

DOI：10.3390/molecules27051598

• jasminin
• TNF-alpha
• RAW264
• 7 cells
• MAPKs
• antiviral
• HSV-1

Previous studies have reported that recombinant tumor necrosis factor (TNF)-alpha has powerful antiviral activity but severe systematic side effects. Jasminin is a common bioactive component found in Chinese herbal medicine beverage "Jasmine Tea ". Here, we report that jasminin-induced endogenous TNF-alpha showed antiviral activity in vitro. The underlying TNF-alpha-inducing action of jasminin was also investigated in RAW264.7 cells. The level of endogenous TNF-alpha stimulated by jasminin was first analyzed by an enzyme-linked immunosorbent assay (ELISA) from the cell culture supernatant of RAW264.7 cells. The supernatants were then collected to investigate the potential antiviral effect against herpes simplex virus 1 (HSV-1). The antiviral effects of jasminin alone or its supernatants were evaluated by a plaque reduction assay. The potential activation of the PI3K-Akt pathway, three main mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-kappa B signaling pathways that induce TNF-alpha production were also investigated. Jasminin induces TNF-alpha protein expression in RAW264.7 cells without additional stimuli 10-fold more than the control. No significant up-expression of type I, II, and III interferons; interleukins 2 and 10; nor TNF-beta were observed by the jasminin stimuli. The supernatants, containing jasminin-induced-TNF-alpha, showed antiviral activity against HSV-1. The jasminin-stimulated cells caused the simultaneous activation of the Akt, MAPKs, and NF-kappa B signal pathways. Furthermore, the pretreatment of the cells with the Akt, MAPKs, and NF-kappa B inhibitors effectively suppressed jasminin-induced TNF-alpha production. Our research provides evidence that endogenous TNF-alpha can be used as a strategy to encounter viral infections. Additionally, the Akt, MAPKs, and NF-kappa B signaling pathways are involved in the TNF-alpha synthesis that induced by jasminin.

• 单位
  南方医科大学