Application of DNA quantitative cytology and high-risk HPV-DNA test in screening cervical lesions

摘要

Objective:To analysis the role of DNA quantitative cytology and high risk HPV-DNA testing in screening of cervical lesions.Methods:We analyzed the data of 5 024 patients who were detected both by DNA ploidy analysis and cervical liquid-based cytology from May 2015 to September 2016 in Jiangsu Province Traditional Chinese Medicine Hospital,among which 1 573 patients were detected by HPV.A total of 146 women were followed by colposcopic examination,where biopsies were taken.Results:Positive rate was 7.74％ in 390 patients with DNA ploidy abnormality and it was 8.1％ in 408 patients detected by TCT,there was no significant difference (P＞0.05).The TCT positive rate were 33.7％,94.7％ and 98.5％ when the 2.5 ≤ DI＜4.5 less than 3 cells,2.5 ≤ DI＜4.5 more than 3 cells,DI ≥ 4.5,respectively,and there was statistically significant difference (P＜0.01).The positive rates of DNA ploidy quantitative analysis of ASC-US,low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion (HSIL) were 45.4％,97.1％ and 100％,respectively.The difference was statistically significant (P＜0.01).The total high-risk HPV infection rate was 24.5％,and with the increase of DI index,the rate of high-risk HPV infection was increasing,with statistical significance (P＜0.01).Histologically positive rate of biopsy was 70.5％ with DNA ploidy while TCT was 65.9％,the difference was not statistically significant (P＞0.05),the total detection rate was 74.6％.Conclusion:DNA quantitative cytology is an effective method of cervical lesion screening.

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