Bacteria Capture and Inactivation with Functionalized Multi-Walled Carbon Nanotubes (MWCNTs)

作者：Ding, Lijun; Wang, Hao; Liu, Dan^*; Zeng, Xin-An; Mao, Yiqin

来源：Journal of Nanoscience and Nanotechnology, 2020, 20(4): 2055-2062.

DOI：10.1166/jnn.2020.17332

• MWCNT Dispersity
• Surface Functionalization
• Incubation
• Antimicrobial Mechanism
• Microbial Affinity

Bacteria capture and inactivation with multi-walled carbon nanotubes (MWCNTs) was investigated in this study. Raw MWCNTs (r-MWCNTs) was oxidized in mixed acid, yielding functionalized MWCNTs (f-MWCNTs) with carboxyl and hydroxyl group. UV-Vis absorption spectra and transmission electron microscope (TEM) shown that surface modifications led to elevated dispersity and stability of f-MWCNTs. The antimicrobial activities of f-MWCNTs to bacterial pathogen Vibrio parahaemolyticus were further explored. Both the colony forming count method and OD growth assay demonstrated that individually dispersed f-MWCNTs showed a higher antibacterial activity compared to r-MWCNT aggregates, suggesting that dispersity of MWCNTs play an important role to antibacterial effect of CNTs. In addition, viability of bacterial cells treated with f-MWCNTs was found to be incubation time-dependent, however no correlation between incubation shaking speed and antibacterial activity of f-MWCNTs was observed. SEM and TEM prove that f-MWCNTs mostly wound around surfaces of V. parahaemolyticus cells instead of piecing into the bacterial cells. This is a convincing proof of physical sorption other than physical punctures, contributing to the possible antimicrobial mechanism of f-MWCNTs to bacterial cells.

• 单位
  广东工业大学