Improving selenium accumulation in broilers using Escherichia coli Nissle 1917 with surface-displayed selenite reductase SerV01

作者：Gong, Yuxin; Wu, Ying; Khan, Aman; Song, Peizhi; Wang, Zhenfei; Ni, Hongyuhang; Ji, Jing; Salama, El-Sayed; Liu, Pu; Li, Xiangkai^*

来源：Food & Function, 2022, 13(8): 4537-4550.

DOI：10.1039/d2fo00206j

• ELEMENTAL SELENIUM
• NANOPARTICLES
• SPECIATION

Selenium levels have a critical impact on livestock and poultry, and selenium nanoparticles (SeNPs) have shown significant efficiency in supplementation. This study identified a high-efficiency selenite reductase, SerV01, in Staphylococcus aureus LZ-01, which can convert Se2O32- to SeNPs. Subsequently, SerV01 was introduced into the intestines of the broilers using the surface display-engineered E. coli Nissle 1917 (EcN). The results showed that the engineered bacteria (EcN-IS) significantly increased the selenium content by 0.87 mg kg(-1), 0.52 mg kg(-1), and 6.10 mg L-1 in the liver, breast muscle, and serum, respectively. With SeNPs + EcN-IS treatment, glutathione peroxidase and thioredoxin reductase levels reached 0.7536 +/- 0.03176 U mu L-1 protein and 2.463 +/- 0.1685 U mu L-1 protein, respectively. With the modified probiotics, the proportion of beneficial intestinal flora increased, with Lactobacillus and Propionibacterium accounting for 75.85% and 0.19%. This technology provides a novel idea to facilitate the exploitation of selenium in broiler diets and improve antioxidant capability.

• 单位
  xk; 兰州大学