Biofunctional Janus particles promote phagocytosis of tumor cells by macrophages

作者：Zhang, Ya-Ru; Luo, Jia-Qi; Li, Jia-Xian; Huang, Qiu-Yue; Shi, Xiao-Xiao; Huang, Yong-Cong; Leong, Kam W.; He, Wei-ling^*; Du, Jin-Zhi^*

来源：Chemical Science, 2020, 11(20): 5323-5327.

DOI：10.1039/d0sc01146k

• MULTIFUNCTIONAL NANORODS
• NANOPARTICLES

Herein, a versatile strategy for the construction of biofunctional Janus particles (JPs) through the combination of Pickering emulsion and copper-free click chemistry is developed for the study of particle-mediated cell-cell interactions. A variety of biomolecules including bovine serum albumin (BSA), ferritin, transferrin (Tf), and anti-signal regulatory protein alpha antibodies (aSIRP alpha), etc., can be incorporated into the Janus platform in a spatially defined manner. JPs consisting of Tf and aSIRP alpha (Tf-SPA1-aSIRP alpha JPs) demonstrate a significantly improved binding affinity to either macrophages or tumor cells compared to their uniformly modified counterparts. More importantly, Tf-SPA1-aSIRP alpha JPs mediate more efficient phagocytosis of tumor cells by macrophages as revealed by real-time high-content confocal microscopy. This study demonstrates the potential advantages of JPs in mediating cell-cell interactions and may contribute to the emerging cancer immunotherapy.

• 单位
  中山大学; 1