Roseicella aerolata sp. nov., isolated from bioaerosols of electronic waste

作者：Qin, Tian; Pan, Yi-min; Ren, Qiao-qiao; Liu, Yu-qi; Chen, Ling-yun; Zhang, Guo-xia^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2022, 72(11): 005602.

DOI：10.1099/ijsem.0.005602

• 16S rRNA gene sequence
• bioaerosols
• genomic taxonomy
• Roseicella aerolata

A novel Gram-stain-negative, non-motile, spherical-shaped and facultatively anaerobic bacterial strain, designated as GB24(T) was isolated from bioaerosols of an E-waste dismantling site in Guiyu, Guangdong Province, South PR China. Growth occurred at 15-40 degrees C (optimum 37 degrees C), pH 5.5-9.5 (optimum 7.0), and up to 0.5% NaCl (w/v) under aerobic conditions, GB24(T) was characterized taxonomically and phylogenetically. The sole isoprenoid quinone detected was ubiquinone-10 (Q-10). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, three unidentified glycolipids, one unidentified phospholipid, and one unidentified aminolipid. Carotenoid pigments were produced. The major cellular fatty acids (> 10% of total fatty acids) were C(17:1 omega)6c (51.5%) and summed feature 8 (13.5%, comprising C-18:1 omega 7c and/or C-18:1 omega 6c). Phylogenetic analysis based on 16S rRNA gene sequence and draft genome grouped strain GB24(T) into the genus Roseicella. GB24(T) was most closely related to Roseicella frigidaeris DB1506T with 97.5% 16S rRNA gene sequence similarity. The draft genome of GB24(T) comprised 6153170 bp with a DNA G+C content of 71.5%. The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH) values between GB24(T) and DB1506(T) were 83.2 % (Ortho ANI), 83.3% [ANI by blast (ANIb)] and 27.0%, respectively. Further genomic analysis of GB24(T) revealed the secondary metabolite clusters of terpene and phosphonate, which indicate the capacity for malleobactin (14 %) and phosphinothricin (6 %) tripeptide production. On the basis of the genotypic, chemotaxonomic and phenotypic results, GB24(T) represents a novel species, for which the name Roseicella aerolata sp. nov. is proposed. The type strain of Roseicella aerolata is GB24(T) (= GDMCC 1.2169(T) = JCM 34449(T)).

• 单位
  6; 南方医科大学