The extracellular non-variable region of Dscam promotes bacterial clearance by promoting phagocytosis of hemocytes in Eriocheir sinensis

作者：Zhang, Xiaoli; Zhang, Xiaona; Zong, Shibo; Shen, Guoqing; Zhao, Yuehong; Li, Weiwei^*; Wang, Qun^*

来源：Developmental and Comparative Immunology, 2023, 138: 104541.

DOI：10.1016/j.dci.2022.104541

• Eriocheir sinensis
• Dscam
• Bacterial clearance
• Extracellular domain
• Non-variable region

As the most typical example of mRNA variable splicing, Down Syndrome Cell Adhesion Molecule (Dscam) can produce a large number of mRNA isomers. It plays an important role not only in the nervous system, but also in the immune system. In Eriocheir sinensis, the extracellular region of Dscam has three variable domains, which can produce 25, 34 and 18 exons and encode the N-terminal region of immunoglobulin (Ig) 2 and Ig3 domains, and the entire Ig7 domain, respectively. In addition to three variable domains, the extracellular non-variable region of Dscam also includes many Ig domains and fibronectin type III (FNIII) domains. However, the role of the extracellular non-variable region function of Dscam in the immune defense of E. sinensis is unclear. In this study, we focused on the role of the extracellular non-variable region of Dscam in crab immune defense. The results indicate that the extracellular non-variable region of Dscam can bind bacteria and has bacteriostatic function. At the same time, the extracellular non-variable region of Dscam can also directly promote bacterial clearance by promoting phagocytosis of hemocytes.