Fish environmental DNA in Tokyo Bay: A feasibility study on the availability of environmental DNA for fisheries

作者：Yuki Hongo^*; Shota Nishijima; Yuki Kanamori; Shuhei Sawayama; Kazuki Yokouchi; Natsuki Kanda; Shiori Oori; Yasuyuki Uto; Mitsuhiro Ishii; Hisanori Mita; Seiji Akimoto; Akane Kusano; Kyu Okabe; Tetsuya Yamazaki; Nobuto Fukuda; Toshihiro Onitsuka; Masayuki Minagawa; Hiroshi Okamura; Hiroaki Kurogi

来源：Regional Studies in Marine Science, 2021, 47: 101950.

DOI：10.1016/j.rsma.2021.101950

• eDNA
• Fish barcoding
• eDNA quantity
• Fisheries
• Monitoring

Environment DNA (eDNA) is a nonlethal sampling method that has potential for evaluating fisheries resources. To evaluate whether eDNA information is useful for fisheries, we collected and analysed eDNA samples from 14 sites in Tokyo Bay from April 2018 to March 2020. Using MiFish universal primers, fish mitochondrial 12S rRNAs were amplified from the eDNA by polymerase chain reaction (PCR) and then sequencing. A total of 535 eDNA samples yielded 44.5 million reads, with 197 of teleosts and 16 of elasmobranchs identified. Of those, 42 identified species were commercially important, with the most abundant being Engraulis japonicus (Japanese anchovy), followed by Konosirus punctatus (dotted gizzard shad) and Lateolabrax japonicusi (Japanese seabass). Mitochondrial cytochrome b copy numbers of K. punctatus and L. japonicus showed a seasonal trend, although catch per unit effort for L. japonicus was not correlated with copy number. Although eDNA is a powerful tool that can detect the presence of fish species from a water sample, caution should be used when interpreting fish biomass based on eDNA dynamics until the method has been better studied.

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