科研之友
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摘要
Main observation and conclusion Inspired by the intricate eukaryotic cell structure, the spontaneously assembling multicompartments capable of spatiotemporally regulated biomacromolecules transportation is still a challenge. Here, a heterogeneous proteinosome-based multicompartment was designed and constructed by combining aqueous two-phase system and Pickering emulsion methods. By loading PEGylated insulin with boronic acid group into the multicompartment and glucose oxidase (GOx) into the innermost microgel domain, a short- or long-term transportation pathway of the loaded insulin was constructed by responding to the concentration of glucose. Moreover, the diminution of dynamic boronic ester bonding, the hindrance of polyethylene oxide micro-chamber, electrostatic interaction and the swelling behavior of hydrogel triggered by GOx/glucose reaction resulted in spatiotemporally controlled multi-pathway transportation mode with the releasing of insulin loaded inside the innermost microgel at high concentration of glucose (10 mg center dot mL(-1)). Specially, by incorporating rhodamine B and fluorescein labelled BSA into the multicompartment, the release procedure of the loaded insulin can be monitored by the fluorescence color-change. Overall, a multimode microcompartment is constructed which is then expected to provide a promising platform for further therapeutic protein, gene and drug delivery, as well as towards the design of complicated cell biomimetics.
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单位南方医科大学; 6
