High-throughput screening and rational design of biofunctionalized surfaces with optimized biocompatibility and antimicrobial activity

作者：Fang, Zhou; Chen, Junjian; Zhu, Ye; Hu, Guansong; Xin, Haoqian; Guo, Kunzhong; Li, Qingtao; Xie, Liangxu; Wang, Lin^*; Shi, Xuetao^*; Wang, Yingjun^*; Mao, Chuanbin^*

来源：Nature Communications, 2021, 12(1): 3757.

DOI：10.1038/s41467-021-23954-8

• OSTEOGENIC DIFFERENTIATION
• CLICK-IMMOBILIZATION
• FUSION PROTEIN
• CELL-ADHESION
• STEM-CELLS
• PEPTIDE
• ANTIBACTERIAL
• SCAFFOLDS
• RGD
• FUNCTIONALIZATION

Peptides are widely used for surface modification to develop improved implants, such as cell adhesion RGD peptide and antimicrobial peptide (AMP). However, it is a daunting challenge to identify an optimized condition with the two peptides showing their intended activities and the parameters for reaching such a condition. Herein, we develop a high-throughput strategy, preparing titanium (Ti) surfaces with a gradient in peptide density by click reaction as a platform, to screen the positions with desired functions. Such positions are corresponding to optimized molecular parameters (peptide densities/ratios) and associated preparation parameters (reaction times/reactant concentrations). These parameters are then extracted to prepare nongradient mono- and dual-peptide functionalized Ti surfaces with desired biocompatibility or/and antimicrobial activity in vitro and in vivo. We also demonstrate this strategy could be extended to other materials. Here, we show that the high-throughput versatile strategy holds great promise for rational design and preparation of functional biomaterial surfaces. Optimizing the concentration of different functional peptides on a surface can be a complex process. Here, the authors report on the use of a click immobilization strategy to create gradients of two different functional peptides on a surface to screen different density functions for rapid optimization.

• 单位
  浙江大学; 江苏大学