ReACT (redox-activated chemical tagging) chemistry enables direct derivatization and fluorescence detection of S-adenosyl-l-homocysteine (SAH)

作者：Lin, Bohong; Xiang, Lingling; Yuan, Zhijun; Hou, Qi; Ruan, Yaoping; Zhang, Jing^*

来源：Organic and Biomolecular Chemistry, 2023, 21(35): 7085-7089.

DOI：10.1039/d3ob01073b

• ARGININE METHYLTRANSFERASE
• PROTEIN
• ADENOSYLHOMOCYSTEINE
• ADENOSYLMETHIONINE
• IMMUNOASSAY
• INHIBITOR
• POTENT
• DISCOVERY
• ENZYMES
• RATIO

S-Adenosyl-l-homocysteine (SAH) is a universal byproduct and product inhibitor of the methyltransferase-catalyzed methylation reaction. Here based on ReACT (redox-activated chemical tagging) chemistry, direct derivatization and fluorescence measurement of SAH were achieved with features such as mild reaction conditions and simple operation.

• 单位
  广州中医药大学; 1