Nanozymatic Biofuel Cell-Enabled Self-Powered Sensing System for a Sensitive Immunoassay

作者：Luo, Xin; Luo, Zhen; Li, Shentian; Fang, Qie; Xu, Weiqing; Wang, Hengjia; Wang, Yongze; Bao, Guang-Ming; Gu, Wenling; Zhu, Chengzhou^*

来源：Analytical Chemistry, 2023, 95(33): 12306-12312.

DOI：10.1021/acs.analchem.3c01576

Self-powered sensing system (SPSS)integrating the enzymaticbiofuelcell and biosensing platform has attracted tremendous interest. However,natural enzymes suffer from the intrinsic drawbacks of enzymes andenzymatic proteins. Nanozymes with enzyme-like activities are theideal alternatives to enzymes, and it is greatly challenging to explorehigh-performance nanozymatic biofuel cell for SPSS. Herein, the advancednanozymatic biofuel cell-enabled SPSS is developed for the sensitivedetection of the prostate-specific antigen (PSA), where Ir singleatoms supported by nitrogen-doped carbon and Au nanozymes serve asthe cathode and anode, respectively. Based on the excellent electrochemicalactivity and stability, the resultant nanozymatic biofuel cell exhibitsa higher power output and open-circuit potential than the Pt/C-basedcounterpart, which is beneficial for the application of SPSS. As aproof of concept, the nanozymatic biofuel cell-enabled SPSS showsa wide detection range of 0.2-500 ng mL(-1) with a detection limit of 62 pg mL(-1) for PSA,which provides new insight into broadening the application scenariosof nanozymes.