The synergetic effect of hierarchical pores and micro-nano bioactive glass on promoting osteogenesis and angiogenesis in vitro

作者：Tian, Ting; Hu, Qing; Shi, Miao; Liu, Cong; Wang, Gang^*; Chen, Xiaofeng^*

来源：Journal of the Mechanical Behavior of Biomedical Materials, 2023, 146: 106093.

DOI：10.1016/j.jmbbm.2023.106093

• Hierarchical pores
• Bioactive glass
• Scaffolds
• Osteogenesis
• Angiogenesis

Hierarchical pores are important structural components of the bone tissue and are closely related to angiogenesis, nutrient transport, and metabolism involved in the repair of a bone defect. Here, we fabricated a composite scaffold having a hierarchical structure, based on micro-nano bioactive glass (MNBG) incorporated into poly (lactic-co-glycolic acid) (PLGA), and with camphene as a pore-forming agent for bone repair. The results showed that camphene formed abundant micropores in the walls of large pores, resulting in hierarchical pore structures ranging from a few microns to a hundred microns. Moreover, there was 2-3 folds increased in compressive modulus and the scaffolds showed a stable degradation rate and a higher degree of apatite crystallization than ordinary porous scaffolds. The results of in vitro studies showed that, when compared to ordinary porous scaffolds, PLGA-MNBG scaffolds with multi-holes could better promote the proliferation of bone marrow mesenchymal stem cells (BMSCs) and the expression of angiogenic marker (CD31) of human umbilical vein endothelial cells (HUVECs).

• 单位
  广州医学院; 1