Eurycomanone (EN) Activates Transcription Factor FoxO by Inhibiting the Insulin Signaling Pathway to Suppress the Development of Spodoptera frugiperda

作者：Zhang, Jie; Wu, Siyu; Wen, Yingjie; Lai, Duo; Kuang, Shizi; Zhang, Ruonan; Xu, Xiaoxia; Jin, Fengliang; Xu, Hanhong; Yu, Xiao-Qiang; Shao, Xuehua^*

来源：Journal of Agricultural and Food Chemistry, 2023, 71(27): 10314-10325.

DOI：10.1021/acs.jafc.3c03324

• Spodoptera frugiperda
• insulin-like signaling
• FoxO
• eurycomanone
• botanical pesticide
• nutrition catabolism

The insulin-like signaling (IIS)pathway is essentialfor insectgrowth and development. In this study, we showed that eurycomanone(EN) is an active compound with growth inhibitory activity against Spodoptera frugiperda larvae. Experiments in cellsand RNA-seq analysis in the midgut showed that EN targeted the IISpathway in S. frugiperda to activatethe transcription factor SfFoxO (S. frugiperda forkhead boxO) to regulate mRNA levels associated with nutrientcatabolism. Additionally, mass spectrometry imaging revealed thatEN was distributed in the larval gut and enriched in the inner membraneof the gut. Immunofluorescence, western blotting, and quantitativereverse transcription-polymerase chain reaction (qRT-PCR) resultsshowed that EN induced program cell death (PCD) in the larvae midgut.Thus, EN targeted the insulin receptor to inhibit the IIS signalingpathway, exerting inhibitory activity on the growth and developmentof S. frugiperda larvae. Our resultssuggest that EN has great potential as a botanical pesticide, andthe IIS signaling pathway may be an effective target for botanicalpesticides.

• 单位
  广东省农业科学院; 华南农业大学