Horseradish Peroxidase Immobilized in Metal Aerogels Boosts Electron Transfer and an Interfacial Reaction for Photoelectrochemical Immunoassay

作者：Li, Jinli; Wang, Hengjia; Liu, Mingwang; Qin, Ying; Fang, Qie; Tan, Rong; Hu, Liuyong^*; Gu, Wenling^*; Zhu, Chengzhou^*

来源：Analytical Chemistry, 2023, 95(26): 10044-10051.

DOI：10.1021/acs.analchem.3c01377

• SIGNAL AMPLIFICATION
• QUANTUM-DOTS
• ENHANCEMENT
• CONVERSION
• TIO2
• BIOSENSOR
• REDUCTION
• ACCEPTOR
• MODE
• GOLD

Photoelectrochemical(PEC) enzymatic biosensors have attractedwidespread attention for their specificity and sensitivity, but thecharge migration between an enzyme and a semiconductor remains uncertain.In this work, horseradish peroxidase (HRP) was successfully immobilizedon ionic liquid-functioned Cu@Cu2O (IL-Cu@Cu2O) aerogels to boost charge transfer and an interfacial redox reaction.The photogenerated electrons flow from the conduction band of Cu2O to HRP under the assistance of Cu and are subsequently capturedby [Fe(CN)(6)](3-) in the electrolyte, whichboosts the PEC response. The improved interfacial catalytic abilityafter the immobilization of HRP is proved by the enhanced redox abilityunder light irradiation. Benefiting from the excellent PEC activityand catalysis reaction of IL-Cu@Cu2O@HRP, an immunoassayplatform was constructed for sensing prostate-specific antigens, whichpresents a wide detection range and a low limit of detection. An in-depthunderstanding of the direct electronic communication between a photoactivematerial and an enzyme for boosted charge transfer and interfacialcatalysis provides a new view for the design of advanced PEC sensingplatforms.

• 单位
  武汉工程大学