Systematic Identification of Microproteins during the Development of Drosophila melanogaster

摘要

Short open reading frame-encoded peptides (SEPs) are microproteins with less than 100 amino acids that play anessential role in the growth and development of organisms. There are plenty of short open reading frames inDrosophila melanogasterthat potentially code polypeptides. We chose 11 time points during the life cycle ofDrosophilato investigate microproteins,particularly those related to development. Finally, we identified a total of 410 microproteins, of which 27 were noncoding RNA-encoded proteins. Of the 410 microproteins, 74 were expressed in all stages from embryo to adults, whereas 300 microproteins wereonly found in one or two time points. Approximately, one-third of the microproteins were not reported previously and 44 wereobtained fromde novosequencing, validated by synthetic peptides. These microproteins are related to the main bioprocesses ofgrowth and development, such as multicellular organism reproduction, postmating behavior, and oviposition. Over half of themicroproteins have predicted functional domains and are conserved across species, suggesting that these microproteins have criticalfunctions infly development. This work enriches theD. melanogasterproteome and provides a significant data resource for growthand development research

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