Non-Invasive and Label-Free On-Chip Impedance Monitoring of Heatstroke

作者：Zhao, Yueli; Fan, Weihua; Liu, Anwei; Pan, Shihua; Xu, Chongxiao; Peng, Hailun; Yin, Bingling; Wang, Xiaodong; Dong, Jianhua; Pan, Zhiguo^*

来源：Biosensors-Basel, 2023, 13(7): 686.

DOI：10.3390/bios13070686

• heatstroke
• biochip
• impedance

Heatstroke (HS) is a life-threatening injury requiring neurocritical care which could lead to central nervous system dysfunction and severe multiple organ failure syndrome. The cell-cell adhesion and cell permeability are two key factors for characterizing HS. To investigate the process of HS, a biochip-based electrical model was proposed and applied to HS. During the process, the value of TEER is associated with cell permeability and C-I which represents cell-cell adhesion decreases that are consistent with the reduction in cell-cell adhesion and cell permeability characterized by proteins (occludin, VE-Cadherin and ZO-1) and RNA level. The results imply that the model can be used to monitor the biological process and other biomedical applications.

• 单位
  中国科学院; 广州中医药大学; 中国医科大学; 南方医科大学