Blood-Glucose-Depleting Hydrogel Dressing as an Activatable Photothermal/Chemodynamic Antibacterial Agent for Healing Diabetic Wounds

作者：Zhu, Wei; Liu, Ya-Qin; Liu, Pei; Cao, Jun; Shen, Ai-Guo^*; Chu, Paul K.^*

来源：ACS applied materials & interfaces, 2023, 15(20): 24162-24174.

DOI：10.1021/acsami.3c03786

• diabetic wound
• smart hydrogel dressing
• glucosedepleting
• activatable antibacterial agent
• photothermaltherapy
• chemodynamic therapy

Poorly healing and nonhealing diabetic wounds are challengingtotreat as the rapid growth of bacteria due to the high local glucosecontent can lead to persistent inflammation and poor angiogenesis.Herein, a smart hydrogel dressing composed of 3,3 ',5,5 '-tetramethylbenzidine/ferrousion/Pluronic F-127/glucose oxidase (TMB/Fe2+/PF127/GOx)is designed and demonstrated to consume blood glucose while acceleratingwound healing by generating antibacterial agents in situ. The loaded GOx degrades blood glucose to provide hydrogen peroxide(H2O2) and gluconic acid to support the Fe2+-based Fenton reaction, and the generated hydroxyl radical(center dot OH) facilitates the oxidation of TMB. The color change fromcolorless to green caused by the oxidation of TMB in the blood glucoserange between 1 and 10 mM can be monitored visually. Simultaneously,this process induced chemodynamic therapy (CDT) by the specific generationof hydroxyl radical (center dot OH) for killing bacteria. Moreover, theoxidized TMB shows strong absorption in the near infrared (NIR) regionso that NIR light can be converted into heat efficiently for photothermaltherapy (PTT). As a result, nearly 100% of Staphylococcusaureus and Escherichia coli are killed bysynergistic PTT/CDT, and the infected skin wounds undergo completerepair along with downregulation of interleukin-6 (IL-6) and upregulationof the vascular endothelial growth factor (VEGF) and matrix metallopeptidase-2(MMP-2). Different from traditional wound dressings that can giverise to secondary injury, the excellent thermosensitive propertiesarising from the sol/gel phase transition render the hydrogel dressingmaterials injectable, self-reparable, and removable on demand. Themultifunctional hydrogel with hypoglycemic, chemodynamic, photothermal,antibacterial, and on-demand thermosensitive properties has immensepotential in the treatment of diabetic wounds.