Identification of Five Gelatins Based on Marker Peptides from Type I Collagen Mass Spectrum in Multiple Reaction Monitoring Mode

作者：Yang, Peilei; Bi, Qirui; Li, Yun; Liao, Jingmei; Ding, Yelin; Huang, Dongdong; Luo, Xiaoxiao; Huang, Yong; Yao, Changliang; Zhang, Jianqing; Wei, Wenlong; Li, Zhenwei; Meng, Jiang^*; Guo, Dean^*

来源：Journal of Agricultural and Food Chemistry, 2023, 71(14): 5851-5860.

DOI：10.1021/acs.jafc.3c00151

• donkey-hide gelatin
• marker peptide
• pseudo-targeted peptidomics
• type I collagen

In this study, a novel pseudo-targeted peptidomics strategy, integrating the transition list generated by an in-house software (Pep-MRMer) and the retention time transfer by high-abundance ion-based retention time calibration (HAI-RT-cal), was developed to screen marker peptides of gelatins from five closely related animal species, including porcine, bovine, horse, mule, and donkey. Five marker peptides were screened from the molecular phenotypic differences of type I collagen. Furthermore, a simple and robust 10 min multiple reaction monitoring (MRM) method was established and performed well in distinguishing different gelatins, particularly in discerning horse-hide gelatin (HHG) and mule-hide gelatin (MHG) from donkey-hide gelatin (DHG). The market investigation revealed the serious adulteration of DHG. Meantime, the pseudo-targeted peptidomics could be used to screen marker peptides of other gelatin foods.

• 单位
  中国科学院; 广东药学院