Electrochemical investigation on the interaction of benzene sulfonyl 5-fluorouracil derivatives with double-stranded DNA and G-quadruplex DNA

作者：HU Quan; ZHANG KeJun; JIN HuiLe; CHEN XiAn; HU MaoLin & WANG Shun Nanomaterials; Chemistry Key Laboratory; Wenzhou University; Wenzhou; China Department of Pharmacology; School of Basic Medicine; Hangzhou Normal University; Hangzhou; China

来源：Science China Chemistry, 2012, 1356-1364.

• anti-tumor activity
• differential pulse voltammetry
• double-stranded DNA
• G-quadruplex DNA
• 5-fluorouracil derivatives

The interaction of double-stranded(ds) and G-quadruplex(G4) DNA with sulfonyl 5-fluorouracil derivatives(5-fluoro-1-(arylsulfonyl) pyrimidine-2,4(1H,3H)-diones) was investigated in this research,in which Au electrodes modified with ds-DNA or G4-DNAs were used as a working electrode.The investigation showed that the binding affinity with G4-DNA was significantly increased when 5-fluorouracil(5-FU) was modified with arylsulfonyl groups.The presence of strong electron-withdrawing groups on benzene sulfonyl 5-FU greatly enhanced the binding selectivity(k G4-DNA /k ds-DNA).Such results provided new insights into the potential connections between the chemical structure of drug candidates and their anticancer activities.