Conversion of Zearalenone to β-Zearalenol and Zearalenone-14,16-diglucoside by Candida parapsilosis ATCC 7330

作者：Pan, Yaoyin; Liu, Chendi; Yang, Jiguo; Tang, Yuqian^*

来源：Food Control, 2022, 131: 108429.

DOI：10.1016/j.foodcont.2021.108429

• Zearalenone
• Candida parapsilosis
• beta-ZOL
• ZEN-14,16-Diglucoside
• Detoxification
• Conversion

Zearalenone (ZEN), mainly produced by Fusarium species in many crops worldwide, is an estrogenic mycotoxin that causes reproductive disorders in livestock. In this study, we developed a different degradation mode by choosing specific microorganisms to catalyze directional reactions to produce less toxic or non-toxic target products. One promising strain, Candida parapsilosis ATCC 7330, with high reductase activity was evaluated for its ability to directly convert ZEN to less toxic beta-Zearalenol (beta-ZOL). Glucose was added to trigger ZEN glucosylation by yeast. Thin layer chromatography (TLC) combined with liquid chromatography-mass spectrometry (LC-MS) revealed that ZEN exposed to C. parapsilosis ATCC 7330 was transformed into beta-ZOL and zearalenone glucosides ([M+H]+ at 643.2581), and it was proposed to be ZEN-14,16-diglucoside according to NMR analysis, which was stable under digestive conditions. Furthermore, kinetic analysis indicated that the ZEN level (20 mu g/mL) decreased by 97% within 5 h, which relied on enzymes on the cell membrane. In addition, C.parcupsilosis ATCC 7330 also exhibited glucosyltransferase activities toward beta-ZOL, alpha-Zearalenol (alpha-ZOL) and Zearalanone (ZAN) with yields of 100%, 76%, and 100%, respectively. Considering the estrogenic toxicity of target metabolites after complex transformation without coenzyme addition, detoxification by specific biodegradable microorganisms may open new opportunities and innovations for mycotoxin detoxification.