A DNA Origami-based Bactericide for Efficient Healing of Infected Wounds

作者：Wu, Tiantian; Wang, Hong; Tian, Run; Guo, Shuang; Liao, Yuhui^*; Liu, Jianbing^*; Ding, Baoquan^*

来源：Angewandte Chemie - International Edition, 2023, 62(46).

DOI：10.1002/anie.202311698

• Bactericide
• DNA Origami
• Drug Delivery
• Infected Wound Healing
• Self-Assembly

Bacteria infection is a significant obstacle in the clinical treatment of exposed wounds facing widespread pathogens. Herein, we report a DNA origami-based bactericide for efficient anti-infection therapy of infected wounds in vivo. In our design, abundant DNAzymes (G4/hemin) can be precisely organized on the DNA origami for controllable generation of reactive oxygen species (ROS) to break bacterial membranes. After the destruction of the membrane, broad-spectrum antibiotic levofloxacin (LEV, loaded in the DNA origami through interaction with DNA duplex) can be easily delivered into the bacteria for successful sterilization. With the incorporation of DNA aptamer targeting bacterial peptidoglycan, the DNA origami-based bactericide can achieve targeted and combined antibacterial therapy for efficiently promoting the healing of infected wounds. This tailored DNA origami-based nanoplatform provides a new strategy for the treatment of infectious diseases in vivo. @@@ A DNA origami-based bactericide with precisely organized bacterial membrane breaker (DNAzymes, G4/hemin) and abundantly loaded broad-spectrum antibiotic (levofloxacin) was constructed for the efficient healing of infected wounds under the guidance of DNA aptamer targeting bacterial peptidoglycan.image

• 单位
  南方医科大学; 国家纳米科学中心; 中国科学院研究生院; 海南大学