Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant

作者：Abdal Aziz Samia Abdallah; Pliego Alfaro Fernando; Que***a Miguel A; Mercado Jose A^*

来源：Journal of Bioscience and Bioengineering, 2006, 101(6): 508-510.

DOI：10.1263/jbb.101.508

• Agrobacterium tumefaciens
• Fragaria x ananassa
• T-DNA transfer
• genetic transformation

We have studied the occurrence of the integration of non-T-DNA sequences in transgenic strawberry plants obtained through Agrobacterium inoculation. DNA from these plants was subjected to PCR amplification of the sequence of the gene trfA, which is located outside the T-DNA. The percentage of trfA-positive plants varied from 40% to 90%, with a mean of 65.7%. Backbone sequences were confirmed by Southern blot analysis.