Optimisation of ultrasonic-assisted extraction and biological activity of total flavonoids from leaves of Murrayae exotica using response surface methodology

作者：Wen, Yao^*; Liu, Manchun; Mai, Xueying

来源：Folia Horticulturae, 2023, 35(1): 135-148.

DOI：10.2478/fhort-2023-0010

• anti-hyperglycaemic activity
• M. exotica leaves
• response surface optimisation
• total flavonoids
• ultrasonic-assisted extraction

Murrayae exotica is a traditional Chinese medicine widely grown in southeast China. A Box-Behnken design of response surface methodology was employed to further optimise ultrasonic-assisted extraction conditions for M. exotica leaves total flavonoids (MELTF). The results showed that the optimised extraction conditions were an ultrasonic power of 240 W, an ultrasonic temperature of 60 degrees C, a solvent concentration of 76%, an ultrasonic time of 55 min and a liquid-solid ratio of 22 mL center dot g(-1). Under these conditions, 8.59 +/- 0.34 mg center dot g(-1) was achieved as the mean experimental value of extraction yield, which amounts to 2.56 times that of ethanol leaching extraction. As compared to MELTF, MELTF after purification (MELPTF) had a higher purity of 9.96%, which was nearly nine times higher than that of MELTF (1.26%). As compared to MELTF, MELPTF had higher a-glucosidase and a-amylase inhibitory activities as well as DPPH center dot and ABTS(center dot+) scavenging activities with IC50 values of 0.021, 0.094, 0.245 and 0.113 mg center dot mL(-1), which are 1.33, 2.12, 3.17 and 1.78 times higher than those of MELTF (0.028, 0.199, 0.777 and 0.201 mg center dot mL(-1)). The study thus demonstrates the eligibility of MELPTF to be considered as a multifunctional bioactive ingredient having potential applications in anti-hyperglycaemic pharmaceutical formulation and as an antioxidant in functional foods.

• 单位
  y; 广东药学院