A two-photon iridium(III) complex probe for sensitive detection of SO2 derivatives in living cell mitochondria

作者：Xin, Haotian; Huang, Yan; Han, Yanyan; Tang, Luyao; Yang, Guiyi; Zhang, Yan; Zhao, Songfang; Wang, Kang-Nan^*; Li, Yibing^*; Cao, Duxia^*

来源：Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy , 2023, 299: 122876.

DOI：10.1016/j.saa.2023.122876

• Iridium(III) complex
• Phosphorescent probe
• Sulfur dioxide derivatives
• Mitochondria

The derivatives of sulfur dioxide (HSO3  ) formed in the biological environment play a vital role in the circulation system. Excessive SO2 derivatives will cause serious damage to the living system. Herein, a two-photon phosphorescent probe based on Ir(III) complex (named as Ir-CN) was designed and synthesized. Ir-CN is extremely selective and sensitive to SO2 derivatives with significant phosphorescent enhancement and increased phosphorescent lifetime. The detection limit of Ir-CN for SO2 derivatives reaches 0.17 & mu;M. More importantly, Ir-CN preferentially accumulates in mitochondria, so bisulfite derivatives can be detected at subcellular level, which enriching the application of metal complex probe in biological detection. In addition, both single-photon and two-photon images can clearly show that Ir-CN is targeted to mitochondria. Benefits from its good biocompatibility, Ir-CN may be used as a reliable tool to detect SO2 derivatives in mitochondrion of living cells.

• 单位
  南方医科大学; 山东大学