科研之友
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摘要
Cherax destructor, a native Australian freshwater crayfish, has been extensively studied for its aquaculture potential, but its growth and immune responses at different temperatures remain unclear. In this study, after 8 weeks of culture at 10 degrees C, 15 degrees C, 20 degrees C, 25 degrees C, and 30 degrees C, the effects of temperature on growth parameters, hepatopancreas histology, antioxidant, and immune indexes in C. destructor were evaluated. The results revealed that the specific growth rate was decreased in the 10 degrees C and 15 degrees C groups. Compared to the 20 degrees C and 25 degrees C groups, the activities of the hepatopancreas damage indicators, aspartate aminotransferase, and alanine aminotransferase (ALT) were both increased in the 30 degrees C group, and the ALT activity was increased in the 10 degrees C and 15 degrees C groups. The activity of antioxidant and immune enzymes showed a decreasing trend, while their corresponding gene expression first increased and then decreased as the temperature decreased. In addition, malondialdehyde content was increased in the 30 degrees C group compared to the 25 degrees C group. These results indicated that the optimal growth temperature of C. destructor was 20-25 degrees C. High temperature may induce oxidative stress which in turn damages the hepatopancreas function of crayfish, thereby reducing antioxidant and immune ability. Low temperature may inhibit the development of the hepatopancreas, thereby affecting its function, resulting in decreased immune defenses and ultimately growth inhibition.
