Comparative proteomic and phenotypic changes of LuxS-mediated hyperosmotic tolerance in Cronobacter malonaticus

作者：Ou, Dexin; Ling, Na^*; Tong, Liaowang; Zhang, Danfeng; Shen, Yizhong; Ye, Yingwang^*

来源：International Journal of Dairy Technology, 2023, 76(4): 927-938.

DOI：10.1111/1471-0307.12990

• biofilm
• Cronobacter malonaticus
• iTRAQ
• LuxS
• osmotic tolerance

Cronobacter malonaticus is a highly hyperosmotic-resistant pathogen classified as Class A pathogen by the Food and Agriculture Organization of the United Nations (FAO). This study explored the roles of LuxS as a quorum-sensing transcription factor in C. malonaticus to tolerate hyperosmotic stress, illustrating that LuxS contributed to survival, biofilm formation and reduced cellular injury when grown with 5.5% NaCl stress. Comparative proteomics between C. malonaticus WT and & UDelta;luxS demonstrated that differentially expressed proteins (DEPs) were abundant in Fe-S cluster proteins and oxidoreductases, suggesting a potential cross-tolerance mediated by LuxS could contribute to resisting hyperosmotic stress. Herein, these findings will provide valuable information on LuxS-mediated osmotic tolerance to controlling C. malonaticus contamination.