Comparative proteomic and phenotypic changes of LuxS-mediated hyperosmotic tolerance in Cronobacter malonaticus

摘要

Cronobacter malonaticus is a highly hyperosmotic-resistant pathogen classified as Class A pathogen by the Food and Agriculture Organization of the United Nations (FAO). This study explored the roles of LuxS as a quorum-sensing transcription factor in C. malonaticus to tolerate hyperosmotic stress, illustrating that LuxS contributed to survival, biofilm formation and reduced cellular injury when grown with 5.5% NaCl stress. Comparative proteomics between C. malonaticus WT and & UDelta;luxS demonstrated that differentially expressed proteins (DEPs) were abundant in Fe-S cluster proteins and oxidoreductases, suggesting a potential cross-tolerance mediated by LuxS could contribute to resisting hyperosmotic stress. Herein, these findings will provide valuable information on LuxS-mediated osmotic tolerance to controlling C. malonaticus contamination.

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