Effects of mechanical force on proliferation and apoptosis of stem cells from human exfoliated deciduous teeth

作者：Liu, Qiong; Qian, Hong^*; Yu, Hao; Ren, Fei; Fang, Jingxiao; Liu, Fang; Liu, Hedi; Liang, Jianying

来源：Clinical Oral Investigations, 2022, 26(8): 5205-5213.

DOI：10.1007/s00784-022-04488-9

• Human deciduous tooth pulp stem cells
• Mechanical force
• Cell proliferation
• Cell apoptosis

Objectives This study was designed to explore the effects of mechanical force on the proliferation, apoptosis, and morphology of stem cells from human exfoliated deciduous tooth pulp (SHEDs). Materials and methods Caries-free stranded deciduous teeth were extracted, and SHEDs were isolated through enzymatic digestion. The cultured SHEDs were subjected to different levels of mechanical stimuli (0, 100, 200, and 300 g) for 7 days (30 min/day) using external centrifugal force. Cell proliferation was evaluated with the CCK-8 assay, and the cell cycle and apoptosis were assessed by flow cytometry. The cell morphology was examined using transmission electron microscopy. Results Cell proliferation assay showed no differences between the three stimulation groups and the control group in day 1 to day 3. From the 4th day, cell proliferation was significantly lower in the mechanical force groups than in the control group, but no significant difference was observed among the three mechanical force groups. Besides, there was no significant difference in cell apoptosis among the four groups for 7 days. On day 7 after stimulation, the SHEDs were shrunken, with significantly increased isochromosome in the nucleus and an increase in lysosomes. Conclusions Mechanical force can inhibit the proliferation and affect morphology of SHEDs, but it has no effect on cell apoptosis.

• 单位
  南方医科大学