Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay

作者：Ruan, Li-Ting; He, Shao-Ying; Zhao, Wan; Cao, Hong-Shuai; Xu, Zhi-Ai; Zhang, Wen^*

来源：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2022, 50(9): 1319-1327.

DOI：10.19756/j.issn.02533820.221099

• Cerium vanadate
• Nanozyme
• Colorimetry
• Glucose
• Total antioxidant capacity

The cerium vanadate (CeVO4)-based nanozyme was synthesized through a hydrothermal synthesis method with arginine as the stabilizer. Due to the change of stabilizer, the ratio of Ce-III to Ce-IV in CeVO4 changed and thus allowed that the nanozyme showed nanoparticle morphology, which was different from that of nanorod prepared with EDTA as stabilizer. In the reaction system of 3,3 ' ,5,5 '-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2),the CeVO4-based nanozyme first catalyzed H2O2 to produce hydroxyl radical (center dot OH),which could oxidize TMB and thus resulted in a colour change of solution from colourless to blue, proving that CeVO4 prepared with arginine had strong peroxidase-like activity and the generation of center dot OH accounted for the outstanding peroxidase-like properties. Based on these features, a colorimetric method was developed for detecting H2O2, glucose and antioxidants. This study not only provided a new direction for the research of CeVO4-based nanozyme, but also developed a fast and simple method for total antioxidant capacity (TAC)assay.