Apoptosis in BEL-7402 cells induced by ruthenium(II) complexes through a ROS-mediated mitochondrial pathway

作者：Jiang Guang Bin; Li Wei; Wang Ji; Han Bing Jie; Lin Gan Jian; Xie Yang Yin; Huang Hong Liang; Liu Yun Jun^*

来源：Transition Metal Chemistry, 2014, 39(8): 849-858.

DOI：10.1007/s11243-014-9867-0

Three Ru(II) polypyridyl complexes [Ru(dmb)(2)(HMSPIP)](ClO4)(2) (1), [Ru(phen)(2)(HMSPIP)](ClO4)(2) (2) and [Ru(dmp)(2)(HMSPIP)](ClO4)(2) (3) were synthesized and characterized. The cytotoxicity in vitro, apoptosis, cell cycle arrest, reactive oxygen species and mitochondrial membrane potential were assayed. The IC50 values of complexes 1, 2 and 3 toward BEL-7402, A549, MG-63 and SK-BR-3 cell lines ranged from 10.9 +/- A 1.6 to 42.0 +/- A 3.4 mu M. Complexes 1, 2 and 3 can effectively induce apoptosis and inhibit the growth of BEL-7402 cells at the G2/M phase. These complexes can enhance the level of reactive oxygen species and induce decrease in the mitochondrial membrane potential. Additionally, complex 2 can down-regulate the expression of antiapoptotic protein of Bcl-2 protein and up-regulate the levels of proapoptotic protein Bim in BEL-7402 cells.

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  广东药学院