Chromosome genome assembly and whole genome sequencing of 110 individuals of Conogethes punctiferalis (Guenee)

作者:Gao, Bojia; Peng, Yan; Jin, Minghui; Zhang, Lei; Han, Xiu; Wu, Chao; Yuan, He; Awawing, Andongma; Zheng, Fangqiang; Li, Xiangdong; Xiao, Yutao*
来源:Scientific Data, 2023, 10(1): 805.
DOI:10.1038/s41597-023-02730-x

摘要

The yellow peach moth, Conogethes punctiferalis, is a highly polyphagous pest widespread in eastern and southern Asia. It demonstrates a unique ability to adapt to rotten host fruits and displays resistance to pathogenic microorganisms, including fungi. However, the lack of available genomic resources presents a challenge in comprehensively understanding the evolution of its innate immune genes. Here, we report a high-quality chromosome-level reference genome for C. punctiferalis utilizing PacBio HiFi sequencing and Hi-C technology. The genome assembly was 494 Mb in length with a contig N50 of 3.25 Mb. We successfully anchored 1,226 contigs to 31 pseudochromosomes. Our BUSCO analysis further demonstrated a gene coverage completeness of 96.3% in the genome assembly. Approximately 43% repeat sequences and 21,663 protein-coding genes were identified. In addition, we resequenced 110 C. punctiferalis individuals from east China, achieving an average coverage of 18.4 x and identifying 5.8 million high-quality SNPs. This work provides a crucial resource for understanding the evolutionary mechanism of C. punctiferalis' innate immune system and will help in developing new antibacterial drugs.

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