Electrochemical behavior of the endocrine disruptor bisphenol A and in situ investigation of its interaction with DNA

作者：Ezoji Hoda; Rahimnejad Mostafa^*; Rahimnejad M^*

来源：Sensors and Actuators, B: Chemical , 2018, 274: 370-380.

DOI：10.1016/j.snb.2018.07.147

• Carbon paste electrode
• Endocrine disruptor
• Bio-interaction
• Cyclic voltammetry
• Carbon nanotubes

A sensitive, eco-friendly voltammetric method was developed to electrochemically monitor endocrine disruptor, bisphenol A (BPA) as well as the bio-interaction between DNA and BPA. The method was based on the electrocatalytic performance of carbon nanotubes and titanium dioxide powder modified carbon paste electrode (CNT/TiO2/CPE). Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and field emission scanning electron microscopy (FESEM) were applied to determine the characteristics of the proposed electrode. The binding mode of BPA and DNA was assessed by differential pulse voltammetry (DPV) and the results were complemented via ultraviolet-visible spectroscopy (UV-vis). Results demonstrated that the interaction of BPA with single-and double stranded DNA were of intercalative mode. The electrochemical parameters such as the number of electron transferred (n) and electron transfer coefficient were also calculated to be 2 and 0.68, respectively. The calibration curve was attained over the concentration range of 0.1-60 mu M with satisfying detection limit of 0.56 mu M.