In vitro biomineralization behaviors and mechanism as well as cell responses of calcium silicate ceramics

作者：Liang, Caixia; Lu, Teliang; He, Fupo; Zhang, Jia; Yue, Ting; Ye, Jiandong^*

来源：Journal of Materials Science, 2022, 57(31): 14655-14667.

DOI：10.1007/s10853-022-07482-4

• APATITE FORMATION

Calcium silicate ceramics have excellent bioactivity and biomineralization performance. In this work, we investigated the effect of different mineralization environments of one time and five times simulated body fluids (x 1 and x 5 SBF) on the apatite mineralization behavior of CaSiO 3 ceramic and explored the mineralization mechanism by soaking them in the x 1 SBF. The CaSiO3 ceramics were found to release Ca2+ and SiO32- ions firstly. The process of ions exchange resulted in the pH increased and the Si-OH groups formed. H+ ions released from the Si-OH groups due to the pH variation, which thereby caused that the surface was negatively charged to recombine with the Ca2+. Meanwhile, the calcium silicate surface layer reformed above the original CaSiO3 ceramic surface. The calcium phosphate layer with different mineral morphologies was induced to be deposited on the reformed calcium silicate surface layer. Importantly, the cytological response results also indicated that a thicker calcium phosphate minerals layer with lamellar-like morphology covered on the CaSiO3 ceramic surface played a more prominent role in up-regulating the expressions of osteogenesis-related genes. @@@ [GRAPHICS] @@@ .

• 单位
  广东省人民医院; 广东工业大学