Binding-induced DNA assembly and its application to yoctomole detection of proteins

作者：Zhang Hongquan; Li Xing Fang; Le X Chris^*

来源：ANALYTICAL CHEMISTRY, 2012, 84(2).

DOI：10.1021/ac203207g

• Affinity ligands
• Binding events
• Biosensing
• Closed-loop structures
• Detection limits
• Diverse applications
• DNA assemblies
• DNA motif
• Molecular targets
• Platelet-derived growth factors
• Prostate specific antigen
• Single tubes
• Streptavidin
• Structural feature
• Ultrasensitive detection
• Washing steps

We describe the binding-induced DNA assembly principle and strategy that enable ultrasensitive detection of molecular targets and potential construction of unique nanostructures/nanoreactors. Two DNA motifs that are conjugated to specific affinity ligands assemble preferentially only when a specific target triggers a binding event. The binding-induced assembly of the DNA motifs results in the formation of a highly stable closed-loop structure, raising the melting temperature (Tm) of the hybrid by >30 °C and enabling effective differentiation of the target-specific assembly from the background. The ability to detect as few as a hundred molecules (yoctomole) of streptavidin, platelet derived growth factor, and prostate specific antigen represents an improvement of detection limits by 103-105-fold over traditional immunoassays. The assay is performed in a single tube, eliminating separation, immobilization, and washing steps of conventional assays. By incorporating unique signaling and structural features into the DNA motifs, we envision diverse applications in biosensing and nanotechnology.