Application on nucleic acid amplification technigues in blood screening for HBV DNA, HCV RNA/HIV-1 RNA

摘要

Objective To investigate and analyze the conditions of seronegative NAT positive donors, and study the feasibility of NAT in blood screening. Methods Seronegative samples were detected by Roche PCR、PCR-CHIP and real-time fluorescence PCR for HBV DNA、HCV RNA and HIV-1 RNA. The positive donors were traced and analyzed by quanti-tative PCR. Results 22 of 131 174 donations were HBV DNA positive,the HBV DNA positive rate was 1:5 962, 15/22 donations were Anti-HBc positive ,the HBV DNA and Anti-HBc positive rate was 1:8 745,14 donors were traced and 8 fol-low-up samples seroconverted, 1 was conformed HCV RNA positive, the positive rate was 1:131 174. Conclusion It is necessary to implement high sensitive HBV and HCV NAT detection in blood screening for blood safety.

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