Application on nucleic acid amplification technigues in blood screening for HBV DNA, HCV RNA/HIV-1 RNA

作者：叶贤林; 李活; 许晓绚; 张红; 曾劲峰; 杨宝成; 朱为刚

来源：中国输血杂志, 2010, 23(1).

• Nucleic Acid Amplification Techniques (NAT)
• Serological test
• Blood Donors
• HBV
• HCV
• HIV
• Seroconversion

Objective To investigate and analyze the conditions of seronegative NAT positive donors, and study the feasibility of NAT in blood screening. Methods Seronegative samples were detected by Roche PCR、PCR-CHIP and real-time fluorescence PCR for HBV DNA、HCV RNA and HIV-1 RNA. The positive donors were traced and analyzed by quanti-tative PCR. Results 22 of 131 174 donations were HBV DNA positive,the HBV DNA positive rate was 1:5 962, 15/22 donations were Anti-HBc positive ,the HBV DNA and Anti-HBc positive rate was 1:8 745,14 donors were traced and 8 fol-low-up samples seroconverted, 1 was conformed HCV RNA positive, the positive rate was 1:131 174. Conclusion It is necessary to implement high sensitive HBV and HCV NAT detection in blood screening for blood safety.

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