Iodide-Enhanced Perovskite Nanozyme-Based Colorimetric Platform for Detection of Urinary Nuclear Matrix Protein 22

作者：Feng, Yangkun; Qu, Xinyu; Peng, Ying; Xu, Xinyu; Zhang, Jie; Wang, Yang; Zhu, Sha; Li, Menglu^*; Li, Chao^*; Feng, Ninghan^*

来源：ACS applied materials & interfaces, 2023, 15(23): 27742-27749.

DOI：10.1021/acsami.3c05437

• nanozyme
• bladder cancer
• perovskitenanocrystals
• nuclear matrix protein 22
• iodide

Inthe last decade, perovskite nanocrystals (PNCs) havebroughtextensive thinking owing to their excellent optical properties. Recently,we have uncovered the peroxidase-like activity of PNCs and used thisfor detecting many small molecules; however, the low enzymatic activitymakes them unsuitable for fluorescence analysis, which is easily disturbedby the autofluorescence of biological media. This greatly limits theirapplication in bioanalysis. Thus, the development of a method to facilelymodulate the activity of PNCs for the instrument-free colorimetricdetection is highly desirable. Herein, we demonstrated an iodide-enhancedperovskite nanozyme-based colorimetric platform for the visual assayof urinary nuclear matrix protein 22 (NMP22), a typical biomarkerfor the diagnosis of bladder cancer. We discovered that halogen couldregulate the activity of perovskite nanozymes through a simple anionreplacement reaction. Experimental analysis suggested that CsPbI3 nanocrystals (NCs) displayed 24-fold higher catalytic efficiencythan classical CsPbBr3 NCs. As a proof-of-concept assay,the CsPbI3 NCs could be explored into an immunoassay forthe detection of NMP22 in clinical urine specimens, resulting in alow detection limit of 0.03 U/mL. This iodide-enhanced immunoassaydeepens our understanding of perovskite nanozymes and also providesgreat potential for bioanalysis.

• 单位
  南京大学; 江南大学; 南通大学