Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode

作者：Wu, Lina^*; Wang, Xinjie; Wu, Chengyuan; Cao, Xizhong; Tang, Taishan; Huang, He^*; Huang, Xingxu^*

来源：Analytica Chimica Acta, 2022, 1221: 340120.

DOI：10.1016/j.aca.2022.340120

• Screen-printedcarbonelectrode
• CRISPR/Cas12a
• SARS-CoV-2
• CeO(2 )nanorods
• Detection

Early and accurate diagnosis of SARS-CoV-2 was crucial for COVID-19 control and urgently required ultra -sensitive and rapid detection methods. CRISPR-based detection systems have great potential for rapid SARS-CoV-2 detection, but detecting ultra-low viral loads remains technically challenging. Here, we report an ultra -sensitive CRISPR/Cas12a-based electrochemical detection system with an electrochemical biosensor, dubbed CRISPR-SPCE, in which the CRISPR ssDNA reporter was immobilized onto a screen-printed carbon electrode. Electrochemical signals are detected due to CRISPR cleavage, giving enhanced detection sensitivity. CRISPR-SPCE enables ultrasensitive SARS-CoV-2 detection, reaching as few as 0.27 copies mu L-1. Moreover, CRISPR-SPCE is also highly specific and inexpensive, providing a fast and simple SARS-CoV-2 assay.