Enzymatic hydrolysis optimization of Paphia undulata and lymphocyte proliferation activity of the isolated peptide fractions

作者：He Xiao Qing; Cao Wen Hong^*; Pan Guang Kun; Yang Liu; Zhang Chao Hua

来源：Journal of the Science of Food and Agriculture, 2015, 95(7): 1544-1553.

DOI：10.1002/jsfa.6859

• Paphia undulata
• response surface methodology
• ultrafiltration
• gel filtration
• lymphocyte proliferation activity
• amino acid sequence

BACKGROUNDThe immune system is important in preventing and controlling various infectious diseases. Immunoactive peptides derived from food proteins could exert immunomodulatory activity without side effects. Paphia undulata with high-protein is suitable for the production of immunoactive peptides. The objective of this study was to optimize the hydrolysis parameters to maximize the immune activity of hydrolysates from Paphia undulata.
RESULTSHydrolysis parameters, including temperature, time and the ratio of alkaline protease to Paphia undulata meat (E/S), were optimized by response surface methodology with a central composite design. The optimum conditions were obtained as follows: temperature, 51 degrees C; time, 3.14h; E/S, 4,321 U g(-1) (Paphia undulata meat). Furthermore, hydrolysates obtained under the optimum conditions were fractionated by ultrafiltration and gel filtration on a Sephadex G-25 column. After two-step isolation, the isolated fractions (P-2 and P-3) including the peptides Pro-His-Thr-Cys, Val-Gly-Try-Thr, Glu-Phe, Leu-Phe, and Glu-Gly-Ala-Lys, Trp-Ile or Trp-Leu, respectively, could significantly enhance lymphocyte proliferation ability.
CONCLUSIONThese results showed that response surface methodology and two-step separation were efficient for recovery of low-molecular-weight peptides with high spleen lymphocyte proliferation activity, and the isolated fractions of Paphia undulata hydrolysates are potential ingredients for development of functional foods.

• 单位
  广东海洋大学