Characterization of phosphofructokinase (PFK) from mud crab Scylla paramamosain and its role in mud crab dicistrovirus-1 proliferation

作者：Jie, Yu-Kun; Luo, Zhi-Ping; Xie, Jia-Wei; Cheng, Chang-Hong; Ma, Hong-Ling; Liu, Guang-Xin; Jiang, Jian-Jun; Deng, Yi-Qin; Feng, Juan; Guo, Zhi-Xun^*

来源：Fish and Shellfish Immunology, 2022, 124: 39-46.

DOI：10.1016/j.fsi.2022.03.042

• Phosphofructokinase
• Scylla paramamosain
• Glycolysis
• MCDV-1

Phosphofructokinase (PFK), the key enzyme of glycolysis, can catalyze the irreversible transphosphorylation of fructose-6-phosphate forming fructose-1, 6-biphosphate. In the present study, a PFK gene from the mud crab Scylla paramamosain, named SpPFK, was cloned and characterized. The full length of SpPFK contained a 5 ' untranslated region (UTR) of 249 bp, an open reading frame of 2,859 bp, and a 3 ' UTR of 1,248 bp. The mRNA of SpPFK was highly expressed in the gill, followed by the hemocytes and muscle. The expression of SpPFK was significantly up-regulated after mud crab dicistrovirus-1 (MCDV-1) infection. Knocking down SpPFK in vivo by RNA interference significantly reduced the expression of lactate dehydrogenase after MCDV-1 infection. Furthermore, silencing of SpPFK in vivo increased the survival rate of mud crabs and decreased the MCDV-1 copies in the gill and hepatopancreas after MCDV-1 infection. All these results suggested that SpPFK could play an important role in the process of MCDV-1 proliferation in mud crab.