Xiao-qing-long-tang ameliorates OVA-induced allergic rhinitis by inhibiting ILC2s through the IL-33/ST2 and JAK/STAT pathways

作者：Zhang, Jia-jun; He, Xue-cheng; Zhou, Min; Liu, Qin-dong; Xu, Wei-zhen; Yan, Ya-jie^*; Ruan, Yan^*

来源：PHYTOMEDICINE, 2023, 119: 155012.

DOI：10.1016/j.phymed.2023.155012

• Xiao-qing-long-tang
• Allergic rhinitis
• ILC2s
• IL-33/ST2 signaling
• JAK/STAT pathway

Background: Allergic rhinitis (AR) is a chronic inflammatory disease of the nasal mucosa that is mediated by immunoglobulin E (IgE). Xiao-qing-long-tang (XQLT) is a traditional Chinese medicine compound that is widely used to treat respiratory diseases such as AR. However, the underlying mechanism of the effect of XQLT on AR remains unclear. @@@ Purpose: To elucidate the effect of XQLT on ovalbumin (OVA)-induced AR and the mechanisms of action. @@@ Methods: The therapeutic efficacy of XQLT was evaluated in a well-established OVA-induced AR mouse model. Nasal symptoms were analyzed, type 2 cytokines and OVA-sIgE levels were measured, nasal mucosa tissues were collected for histological analysis, and the changes of Group 2 innate lymphoid cells (ILC2s) and the IL-33/ST2 and JAK/STAT signaling pathways in the nasal mucosa were observed. @@@ Results: XQLT significantly alleviated the nasal symptoms and histological damage to the nasal mucosa in AR mice, and reduced the levels of type 2 cytokines and OVA-sIgE. In addition, after XQLT treatment, the numbers of ILC2s in the nasal mucosa of AR mice were reduced, and the mRNA levels of the transcription factors GATA3 and ROR-alpha were decreased. Moreover, IL-33/ST2 signaling pathway was inhibited. The costimulatory cytokine associated JAK/STAT signaling pathway was also inhibited in ILC2s. @@@ Conclusion: Our study demonstrated that XQLT regulated ILC2s through the IL-33/ST2 and JAK/STAT pathways to ameliorate type 2 inflammation in OVA-induced AR. These findings suggest that XQLT might be used to treat AR.

• 单位
  1; 南方医科大学; y; 广州中医药大学