Optimization of extraction process of total flavonoids from Cortex Lycii and its biological activities

作者：Wen, Yao^*; Zeng, Xiang; Tan, Hongyu; Liu, Bing^*

来源：Biomass Conversion and Biorefinery, 2023.

DOI：10.1007/s13399-023-03768-y

• Cortex Lycii
• Total flavonoids
• Response surface design
• Hypoglycemic activity
• Antioxidant activity

To research the optimization of ultrasonic-assisted extraction of total flavonoids from Cortex Lycii and its biological activities. Box-Behnken design experiment was used to optimize the extraction process, and AB-8 macroporous resin was used for purification. Its biological activities were studied by the inhibition rate of alpha-glucosidase and alpha- amylase and the scavenging rate of DPPH and ABTS. The optimal extraction parameters of total flavonoids from Cortex Lycii were as follows: the extraction temperature was 63 degrees C, the ethanol volume fraction was 71%, the solid-liquid ratio was 28 mL/g, the extraction time was 61 min. Under this optimal condition, the yield of total flavonoids was 1.674 mg/g, which was consistent with the predicted value of 1.637 mg/g, and was 5.85 times higher than that of the traditional water extraction method. The inhibitory rate (IC50) of total flavonoids extract from Cortex Lycii on alpha-glucosidase was 3 mu g/mL and on alpha-amylase was 5 mu g/mL. The scavenging rate (IC50) of DPPH radical was 0.648 mg/mL and the scavenging rate (IC50) of ABTS radical IC50 was 0.012 mg/mL. The optimized extraction process with response surface design was reliable, which could be used for the extraction of total flavonoids from Cortex Lycii. It has strong hypoglycemic and antioxidant activities and has a good prospect for future utilization and development.

• 单位
  广东药学院; y; 1