Validation and forensic application of a new 19 X-STR loci multiplex system

Authors：Xiao, Cheng; Yang, Xingyi; Liu, Hong; Liu, Changhui; Yu, Zhonghao; Chen, Ling^*; Liu, Chao

Source：Legal Medicine, 2021, 53: 101957.

DOI：10.1016/j.legalmed.2021.101957

• Forensic Genetics
• X-STR loci
• Kinship analysis
• Validation

The MicroreaderTM 19X Direct ID System was a newly developed multiplex PCR kit, which could detect 19 Xchromosomal STR loci (DXS6795, DXS9907, DXS6803, GATA172D05, DXS6807, GATA31E08, DXS7423, DXS6810, DXS101, DXS9902, DXS7133, DXS6800, DXS981, DXS10162, DXS6809, DXS10135, HPRTB, GATA165B12, DXS10079) and the sex determination locus of AMEL simultaneously. Different from other X-STR multiplex PCR kits, no linkage groups are included in this system, so the likelihood ratios could be calculated without the consideration of linkage groups. In this study, PCR conditions, sensitivity, species specificity, stability, DNA mixtures, concordance, stutter, sizing precision and population studies were conducted according to the SWGDAM developmental validation guidelines. The results indicated that this new X-STRs multiplex system was an efficient and reliable detection system, which could facilitate human kinship analysis and identification testing, as a powerful supplementary to autosomal STR kits.

• Institution
  南方医科大学