Heterogeneity of Red Blood Cell Deformability Caused by Lipopolysaccharide based on a Microfluidic Chip

作者：Chen, Yue; Liu, Zhen; Shao, Huaze; Xu, Jun; Li, Jincheng; Chen, Haoyi; Zhou, Chunqiong; Liu, Lihong^*

来源：CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2023, 24(14): 1795-1802.

DOI：10.2174/1389201024666230330134044

• Lipopolysaccharide
• red blood cell deformability
• microfluidic chip
• inter-individual variability
• oxidative damage
• energy metabolism

Introduction: Alterations in red blood cell deformability (RBC-df) provide important information for the diagnosis of various diseases.Aim: We evaluated individual differences of lipopolysaccharide (LPS)-induced oxidative damage of RBC-df and analyzed the correlation between RBC-df and biochemical parameters.Methods: A microfluidic chip was developed to detect inter-individual variability of different concentrations of LPS-induced oxidative damage of RBC-df in 9 healthy volunteers. The relationships between various biochemical indicators (Na+-K+-ATPase activity, lipid peroxide (LPO) content, glutathione peroxidase (GSH-PX) activity, catalase (CAT) activity, superoxide dismutase (SOD) activity, adenosine triphosphate (ATP) content, and hemoglobin (HB) content) and RBCsdf were investigated.Results: The obvious inter-individual variability of LPS-induced oxidative damage of RBC-df was revealed. The Na+-K+-ATPase activity, LPO content, GSH-PX activity, and CAT activity of RBCs showed significant correlations with RBC-df (P < 0.05).Conclusion: Oxidative damage and energy metabolism are the critical factors of RBC-df impairment induced by LPS, and the individual dependence on RBC-df is an important indicator for the treatment of infection-associated sepsis since antibiotics can kill pathogenic bacteria, which results in the release of LPS from the cell wall.

• 单位
  南方医科大学