Immunostimulant In Situ Fibrin Gel for Post-operative Glioblastoma Treatment by Macrophage Reprogramming and Photo-Chemo- Immunotherapy

作者：Zhang, Ruotian; Ye, Yicheng; Wu, Jianing; Gao, Junbin; Huang, Weichang; Qin, Hanfeng; Tian, Hao; Han, Mingyang; Zhao, Boyan; Sun, Zhenying; Chen, Xin; Dong, Xingli; Liu, Kun; Liu, Chang^*; Tu, Yingfeng^*; Zhao, Shiguang^*

来源：ACS applied materials & interfaces, 2023, 15(14): 17627-17640.

DOI：10.1021/acsami.3c00468

• glioblastoma
• mesoporous polydopamine
• nanovesicle
• tumor-associated macrophage polarization
• fibrin gel

Tumor recurrence remains the leading cause of treatment failure following surgical resection of glioblastoma (GBM). M2-like tumor-associated macrophages (TAMs) infiltrat -ing the tumor tissue promote tumor progression and seriously impair the efficacy of chemotherapy and immunotherapy. In addition, designing drugs capable of crossing the blood-brain barrier and eliciting the applicable organic response is an ambitious challenge. Here, we propose an injectable nanoparticle-hydrogel system that uses doxorubicin (DOX)-loaded mesoporous polydop-amine (MPDA) nanoparticles encapsulated in M1 macrophage-derived nanovesicles (M1NVs) as effectors and fibrin hydrogels as in situ delivery vehicles. In vivo fluorescence imaging shows that the hydrogel system triggers photo-chemo-immunotherapy to destroy remaining tumor cells when delivered to the tumor cavity of a model of subtotal GBM resection. Concomitantly, the result of flow cytometry indicated that M1NVs comprehensively improved the immune microenvironment by reprogramming M2-like TAMs to M1-like TAMs. This hydrogel system combined with a near-infrared laser effectively promoted the continuous infiltration of T cells, restored T cell effector function, inhibited the infiltration of myeloid-derived suppressor cells and regulatory T cells, and thereby exhibited a strong antitumor immune response and significantly inhibited tumor growth. Hence, MPDA-DOX-NVs@Gel (MD-NVs@Gel) presents a unique clinical strategy for the treatment of GBM recurrence.

• 单位
  哈尔滨医科大学; 南方医科大学; 1