In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer

作者：Ohuchi; Shoji

来源：Journal of Bioscience and Bioengineering, 2012, 114(1).

DOI：10.1016/j.jbiosc.2012.02.017

• Aptamers
• In-vitro
• Promoter mutation
• RNA aptamer
• RNA polymerase

In vitro selection system for the isolation of bacteriophage promoters was developed. Employing the system, one round of the selection achieved ~20-30 fold enrichment of an active promoter from mixtures with an inactive one. In addition, active T7 promoter variants were successfully selected from a library with six randomized bases.

• 单位
  university of tokyo; 1; 6