Luteoloside Inhibits Proliferation of Human Chronic Myeloid Leukemia K562 Cells by Inducing G2/M Phase Cell Cycle Arrest and Apoptosis

作者：Shao Jun Li^*; Liang Hai Rong; Dai Juan Xiu

来源：Tropical Journal of Pharmaceutical Research, 2016, 15(1): 39-45.

DOI：10.4314/tjpr.v15i1.6

• Luteoloside
• Myeloid leukemia
• Proliferation
• Cell cycle arrest
• Apoptosis
• Anticancer

Purpose: To investigate the effects of luteoloside on the proliferation of human chronic myeloid leukemia K562 cells and whether luteoloside induces cell cycle arrest and apoptosis in K562 cells.
Methods: Luteoloside's cytotoxicity was assessed using a cell counting kit. Cell cycle distribution was analysed by flow cytometry after propidium iodide (PI) staining. Cell apoptosis was assayed with apoptosis detection kit and Hoechst staining followed by observation under a fluorescence microscope. The expression of cell cycle-and apoptosis-related proteins was examined by Western blot analysis.
Results: Luteoloside inhibited the proliferation of K562 cells in a dose-and time-dependent manner (IC50 = 30.7 mu M) with less toxicity in a normal human cell line (IC50 = 91.8 mu M). Moreover, antiproliferative effect of luteoloside was accompanied with G2/M phase arrest(p < 0.05 or p < 0.01) and apoptosis(p < 0.01 or p < 0.001). Further studies revealed that the expression level of cyclinB1 was down-regulated by luteoloside treatment. Furthermore, luteoloside treatment also increased proapoptotic protein Bax expression and decreased anti-apoptotic protein Bcl-2 expression.
Conclusion: These results suggest that the inhibitory effect of luteoloside on K562 cell proliferation is associated with inducing

• 单位
  广东医学院