DNA Biosensor Based on Large Area of Gold Particles on Aligned Carbon Nanotubes for Detection of Promyelocytic Leukaemia/Retinoic Acid Receptor αFusion Gene

Authors：杨丽珠; 朱婧; 章仁毅; 马丹琦; 何品刚; 方禹之

Source：分析化学, 2016, (3).

DOI：10.11895/j.issn.0253-3820.150773

• Aligned carbon nanotubes
• Gold particles
• Acute promyelocytic leukemia
• DNA biosensor
• Differential pulse anodic stripping voltammetry

A novel electrochemical DNA biosensor was fabricated based on the large area of gold particles on the aligned carbon nanotubes for the detection of promyelocytic leukaemia/retinoic acid receptor α ( PML/RARα) fusion gene in acute promyelocytic leukemia. Firstly, the thiol-modified single-stranded DNA ( ssDNA, probe 1) was immobilized on the gold particles sputtered on the aligned carbon nanotubes electrode by self-assembly technique. Then, amino-modified ssDNA and carboxyl-modified CdTe quantum dots ( QDs) were combined to get CdTe-modified DNA probe through an amidization reaction. After hybridization with target DNA, a sandwich-type assay structure was formed. Finally, the CdTe quantum dots captured on the electrode surface were detected by differential pulse anodic stripping voltammetry. The change of peak current of Cd2+ on the electrode was found to be linear with the logarithm of target DNA concentration in the range of 1. 0×10-12 mol/L to 1. 0×10-8 mol/L. The Linear equation was ipa(μA)=1. 626+0. 132lgC (mol/L) (the correlation coefficient R was 0. 996) and the detection limit was 4. 0×10-13 mol/L (3σ). The fabricated DNA biosensor exhibited excellent reproducibility and stability.

• Institution
  温州医学院