Simultaneous Determination of Four Major Constituents in Citri Grandis Exocarpium by HPLC-DAD

Authors：Yu X X; Liu Q D; Wu J W; Liang Z K; Zhao M Q; Xu X J^*

Source：Acta Chromatographicae, 2016, 28(1): 129-143.

DOI：10.1556/1326.2016.28.1.10

• determination
• Citri Grandis Exocarpium
• HPLC-DAD

Citri Grandis Exocarpium (CGE) is a traditional Chinese medicine with a variety of biological activities. For efficient quality control of CGE, a simple, rapid, and accurate high-performance liquid chromatographic (HPLC) method was developed for simultaneous determination of four main compounds (naringin, rhoifolin, meranzin hydrate, and isoimperatorin) in this herb. These four compounds were separated on a C-18 column by gradient elution with methanol and water. The flow rate was 1.0 mL min(-1), and the detection wavelength was 324 nm. The recoveries of the method ranged from 96.32% to 103.71%, and good linear relationships (r(2) > 0.9998) over relative wide concentration ranges were obtained. Then this validated method was successfully applied to the analysis of nine batches of CGE samples.

• Institution
  中山大学